TOOS (N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt), as an important chromogen substrate, plays a crucial role in various biochemical experiments. Due to the unique structure of TOOS reagent, there are many reaction steps involved in the synthesis process and multiple factors need to be controlled. This time, we will explore in detail the properties, synthesis principles, and precautions during the synthesis process of TOOS.
The properties of TOOS
TOOS, as a highly water-soluble aniline derivative, has excellent solubility and stability. It can maintain a high concentration in aqueous solutions and is not prone to decomposition or precipitation. This characteristic enables TOOS to serve as an ideal chromogenic substrate reagent in biochemical experiments, for detecting the content and activity of various biomolecules.
In addition, TOOS also has advantages such as high sensitivity and fast reaction speed. In enzymatic reactions, TOOS can quickly react with the produced hydrogen peroxide (H2O2) to generate red quinone imine compounds with strong absorbance. This compound can be quantitatively measured using a spectrophotometer, thereby achieving accurate detection of the target substance.
The synthesis principle of TOOS
The synthesis method of TOOS is relatively simple, usually obtained by reacting the corresponding aniline derivatives with sulfonation reagents. The specific steps include: first, diazotizing aniline derivatives with sodium nitrite to generate diazonium salts; Then, react the diazonium salt with the sulfonation reagent to obtain the sulfonation product; Finally, the target product TOOS is obtained through steps such as reduction and neutralization.
During the synthesis process, it is necessary to pay attention to controlling reaction conditions, such as temperature, pH value, etc., to ensure the smooth progress of the reaction and the purity of the product. In addition, strict purification and characterization of the product are required to ensure that its quality and performance meet the application requirements.
3、 Notes during the synthesis process of TOOS
1. Temperature monitoring: Sulfonation reactions often occur within the range of low to medium temperatures. Excessive temperatures may lead to increased side reactions, product decomposition, or intensified equipment corrosion. Strictly control the reaction temperature and maintain a suitable reaction environment through methods such as jacket cooling or constant temperature water bath.
2. PH regulation: Monitor the pH value of the reaction system in a timely manner, and add buffering agents if necessary to maintain the stability of the acidity and alkalinity of the reaction medium, to prevent excessive acidification or alkalization from causing adverse effects on the reaction process and equipment.
3. Mixing speed: Maintain an appropriate mixing speed to promote uniform material mixing and avoid local overheating or insufficient reaction. At the same time, consider changes in the viscosity of the reaction material and adjust the stirring intensity in a timely manner.
4. Catalyst selection and dosage: Reasonably select catalysts (such as sulfuric acid, p-toluenesulfonic acid, etc.) and precisely control their dosage to improve the alkylation reaction rate and selectivity, and reduce the generation of by-products.
5. Reaction monitoring: By regularly sampling and analyzing, track the reaction process, such as monitoring the generation of intermediates and target products through thin layer chromatography (TLC) or gas chromatography (GC), and terminate the reaction in a timely manner.
6. Timing and rate of alkaline substance addition: The salt formation reaction should be carried out after the alkylation reaction is completed to avoid premature neutralization and loss of sulfonation intermediates. Alkaline substances should be slowly added in batches to control the reaction rate and system temperature, and to prevent splashing or equipment overpressure caused by local violent reactions.
7. Experimental records and data analysis: Detailed experimental records and data analysis are essential in the process of TOOS synthesis experiments. The experimental records should include information such as the amount of raw materials used, reaction conditions, and experimental steps for subsequent analysis and summary.
In summary, the precautions during the synthesis of TOOS include temperature monitoring, pH adjustment, stirring speed, catalyst selection and dosage, and other aspects. Only by strictly adhering to these precautions can we ensure the success and safety of the synthesis experiment and obtain high-quality TOOS products. Therefore, when conducting TOOS synthesis experiments, it is essential to maintain caution and focus, follow experimental specifications, and ensure the accuracy and reliability of the experimental results. Desheng is a manufacturer of The new Trinder's reagents and can provide a series of reagents such as TOOS. Independently developed and produced, with small inter batch differences and sensitive reactions, it has important value in clinical diagnosis. If you need to make a purchase, please feel free to contact us for consultation and ordering!
