Cas No 29915-38-6 Good Buffer Solutions biological buffer TAPS

BUFFER SOLUTIONS;GOOD'S BUFFER, TAPS; CAS#29915-38-6;WHITE CRYSTAL POWDER PURITY>99%

Synthesis process of biological buffer TAPS

TAPS, its full name is N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid, is a zwitterionic buffer, widely used in the fields of biochemistry and molecular biology. It is part of the Tris series of buffers, with a pH buffer range of 7.7-9.1, soluble in water (25g/50ml). TAPS, as a commonly used buffer system in DNA screening systems, can also be used as a buffer component of RNA samples. It is suitable for electron transfer and phosphorylation studies of chloroplast thin-layer preparations, and protects oxyhemoglobin from oxidation to high iron during freeze-drying. Hemoglobin can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.
Chinese name N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid molecular weight 243.28
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The chemical structure of TAPS

Preparation method of taps solution (about 1-2l)

(1) Prepare 0.1M solution (a): taps 24.328g/deionized water 1000ml

(2) Prepare 0.1M NaOH solution (b): NaOH 4G / deionized water 1000ml

Abbreviation TAPS Molecular formula C7H17NO6S
CAS# 29915-38-6 Storage conditions Room temperature, avoid light and moisture
Melting point 230-235℃ Color white
At present, there are few reports on the synthesis process of TAPS, and Chen Guie et al. have studied the synthesis method of TAPS in detail. They use tris (Tris) and 1,3-propane sultone (1,3-PS) as raw materials to react in alcohol solvents. The operation process is as follows:
1. Weigh an equimolar amount of Tris and 1,3-PS, and take an appropriate amount of alcohol solvent into a 250ml three-necked flask with condensing reflux, heating and refluxing while stirring;
2. After reacting for a period of time, stop the reaction; at room temperature, let the reacted solution stand and cool to room temperature, and then filter under reduced pressure. The TAPS coarse filter cake is washed with an appropriate amount of absolute ethanol for 2 to 3 times, and TAPS is taken out The filter cake is placed in a vacuum drying oven for drying, and the filtrate filtered under reduced pressure is recovered for use.
The main reaction formula is as follows:

In the above synthesis process, the reaction time, different alcohol solvents and the recycling of reaction solvents all have a certain degree of influence on the yield and purity of TAPS. The experimental results show that the reaction time is controlled at 6 hours, and the yield is the highest, reaching 70.3%. Continue to extend the reaction time, and the yield will decrease slightly; for the investigated ethanol, n-propanol, isopropanol and n-butanol reaction solvents, n-butanol As the reaction solvent, the yield and purity of TAPS are the highest, 70.3% and 94.36%, respectively; in the reaction process, the recycling of the reaction solvent is beneficial to improve the yield and purity of TAPS.
In summary, using Tris and 1,3-PS as raw materials to synthesize TAPS, the reaction time is controlled at 6 hours, and n-butanol is used as the solvent, and recycled during the reaction process, the yield and purity of TAPS obtained are the best.
Desheng Technology specializes in R&D and production of various biological buffers, including Tris buffer, Tris-HCl buffer, Bicine buffer, Caps buffer, Mops, Taps, EPPS, etc. The TAPS developed and produced by our company has a purity of ≥99%, Good water solubility, stable process, can ensure the appearance of the product is pure white crystals, professional inquiries are welcome