Cas No 29915-38-6 C7H17NO6S Good's Buffer TAPS Biological Buffer Powder High Purity

Cas No 29915-38-6 C7H17NO6S Good's Buffer TAPS Biological buffer Powder with High Purity

TAPS, its full name is N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid, is a zwitterionic buffer, widely used in the fields of biochemistry and molecular biology. It is part of the Tris series of buffers, with a pH buffer range of 7.7-9.1, soluble in water (25g/50ml). TAPS, as a commonly used buffer system in DNA screening systems, can also be used as a buffer component of RNA samples. It is suitable for electron transfer and phosphorylation studies of chloroplast thin-layer preparations, and protects oxyhemoglobin from oxidation to high iron during freeze-drying. Hemoglobin can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.
Chinese name N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid molecular weight 243.28

Preparation method of taps solution (about 1-2l)

(1) Prepare 0.1M solution (a): taps 24.328g/deionized water 1000ml

(2) Prepare 0.1M NaOH solution (b): NaOH 4G / deionized water 1000ml

Abbreviation TAPS Molecular formula C7H17NO6S
CAS# 29915-38-6 Storage conditions Room temperature, avoid light and moisture
Melting point 230-235℃ Color white
At present, there are few reports on the synthesis process of TAPS, and Chen Guie et al. have studied the synthesis method of TAPS in detail. They use tris (Tris) and 1,3-propane sultone (1,3-PS) as raw materials to react in alcohol solvents. The operation process is as follows:
1. Weigh an equimolar amount of Tris and 1,3-PS, and take an appropriate amount of alcohol solvent into a 250ml three-necked flask with condensing reflux, heating and refluxing while stirring;
2. After reacting for a period of time, stop the reaction; at room temperature, let the reacted solution stand and cool to room temperature, and then filter under reduced pressure. The TAPS coarse filter cake is washed with an appropriate amount of absolute ethanol for 2 to 3 times, and TAPS is taken out The filter cake is placed in a vacuum drying oven for drying, and the filtrate filtered under reduced pressure is recovered for use.
The main reaction formula is as follows:

Storage Conditions

Room temperature, light-proof and moisture-proof

Use

As a buffer system commonly used in DNA screening system, it can also be used as a buffer component of RNA samples. It is suitable for the study of electron transfer and phosphorylation of thin-layer chloroplast preparation. It can protect oxyhemoglobin from oxidizing to methemoglobin during freeze-drying process, and can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.

Advantage

The purity (> 99%) is water-soluble, the process is stable, and the appearance of the product can be guaranteed to be pure white crystal powder.

Biological buffer for separation of dyes in chromatography

Chromatography is a laboratory technique, which is usually used to separate and purify proteins. In this process, the separation ability of the system is directly related to the change of pH. For example, if the pH value of the mobile phase (solvent) is close to pKa, a small change in the pH value will seriously affect the retention rate, thus leading to separation. Due to the retention of ionizable compounds.The mobile phase pH is particularly sensitive, so a buffer should be added to the system to control this variable.

Based on the most relevant literature on chromatography, Desheng's researchers found that although Tris and MES are generally considered to be the best choice, other buffers include taps, which have also been used in cation exchange chromatography, anion exchange chromatography, high performance liquid chromatography (HPLC) and other similar technologies.

One of the most suitable biological buffers for cell culture

The most important characteristics of good's buffer.One is that they are not toxic to cells. Therefore, these chemicals are widely used in cell culture to keep the experiment,The pH value of is under control. Due to their special functions, many good's Buffers / biological buffers.It is considered to be an ideal choice for cell separation, cell culture, enzyme analysis and many other biochemical applications.

Desheng's researchers found that protonated sulfamate compounds directly inhibited the activity of connexin channels.In good'sph buffer (MES (4-morpholine ethane sulfonic acid), HEPES and taps (3 - {[2-hydroxy-1,1-In bis (hydroxymethyl) ethyl] amino] - 1), the pH dependent activity of the linker channel demonstrates this.Propane sulfonic acid), they have a common sulfamate moiety, and there is no sulfamate.Part (of the pH buffer does not have pH dependent channel activity.Therefore, taps is more used for cell culture Experiments on flagellate algae in the substrate.

Preparation method of taps solution (about 1-2l)

(1) Prepare 0.1M solution (a): taps 24.328g/deionized water 1000ml

(2) Prepare 0.1M NaOH solution (b): NaOH 4G / deionized water 1000ml

* temperature 20 degrees.

* use a pH meter if you need to adjust to a specific pH.

* do not want to join Na, please use KOH.