Composition Of HEPES Buffer CAS 7365-45-9 For Biochemical Experiments

In the research of biochemistry and molecular biology, buffer is one of the indispensable and important reagents. HEPES buffer, as an excellent biological buffer, is widely used in the fields of biology and medicine. What substances are it actually composed of? In what areas can it be applied? Below, we will focus on analyzing the composition of HEPES buffer and its important role in biological experiments.

HEPES buffer parameters:

Properties of HEPES buffer

HEPES buffer is an effective biological buffer with weak acidity and alkalinity within the physiological pH range, typically operating within the pH range of 6.8 to 8.2. This makes HEPES buffer very suitable for cell culture and biomolecular research, because it has little impact on biological systems and will not cause cytotoxicity or Protein structure changes.

hepes powder

Application of HEPES buffer

HEPES buffer has a wide range of applications in biological experiments, mainly including the following:

(1) Cell culture: HEPES buffer is commonly used in the preparation of cell culture media to maintain the culture medium at a constant pH value and provide a stable cell environment.

(2) Enzyme activity research: It can serve as a buffer medium for enzyme activity measurement, maintaining enzyme activity within an appropriate pH range.

(3) Polymerase chain reaction (PCR): HEPES buffer is used as buffer medium in PCR reaction to ensure the stability and accuracy of the reaction system.

HEPES buffer, as an important biological buffer, has been widely used in biological and medical research due to its unique chemical structure and applicability. As a professional manufacturer of buffering agents, Desheng can provide buffering agent raw materials with a purity of up to 99%, suitable for various biochemical experiments, and has a wide range of product types, making one-stop selection time-saving and worry free. If you have any related needs, please feel free to contact us for purchase!